Abstract

Research Article

Evaluation of the effects of Leech Salivary Extract (LSE) on Haematological parameters in Rats

Hausatu Babayi*, Ojo Olufunmilola Praise, Labake Fadipe Ajoke, Israel Kayode Olayemi, Elisha Baba and Oladosun Oluwale Peter

Published: 19 January, 2018 | Volume 2 - Issue 1 | Pages: 006-014

The effects of Leech Salivary Extract (LSE) on some haematological, immunological and organ weight parameters in rats, during a twenty eight days oral administration of 25, 50 and 100 mg/kg body weight doses, was investigated. LD50 and sub chronic toxicity was determined using standard methods. The oral LD50 was above 5000mg/kgbw. Oral administration of LSE (25mg/kgbw, 50mg/kgbw, 100mg/kgbw) for 28days had no significant (p>0.05) effect on the differential white blood cells (lymphocytes, monocytes, basophils, neutrophils, eosinophils), red blood cell indices (RBC count, PCV, HB, platelets, MCHC and MCH), feed intake, body weight gain and relative organ weight of lung, heart, liver, kidney, spleen and stomach of rats. However, the LSE evoked a significant (p>0.05) increase in the level of MCV in treated rats compared to the control. These results, indicating low toxicity and no negative significant effects of LSE on haemato-immunological indices in rats, suggest that the extract is safe for development and use as therapeutic for managing clinical conditions.

Read Full Article HTML DOI: 10.29328/journal.jhcr.1001006 Cite this Article Read Full Article PDF

Keywords:

Blood metabolites; Leech Salivary Extract; Relative organ weight; Sub-chronic toxicity; Wister rats

References

  1. Odeghe O, Uwakwe AA, Monago C.C. Some biochemical and haematological studies on the methanolic extract of Anthocleista grandiflora stem bark. International Journal of Applied Science and Technology.2012; 2: 58-65. Ref.: https://goo.gl/rAXKCC
  2. Probst R, Lim J, Bird D, Pole G, Sato A, et al. Gender Differences in Blood Volume of Conscious Sprague-Dawley Rats. J Am Assoc Lab Anim Sci. 2006; 45: 49-52. Ref.: https://goo.gl/4diu41
  3. VanMeter K, Hubert R. Microbiology for the healthcare professional (2nd Ed.). St Loius, MO: Elsevier. 2015; 263-270. Ref.: https://goo.gl/ykJvYa
  4. Whitaker I.S, Rao J, Izadi D, Batter P.E. Historical Article: Hirudo medicinalis ancient origins of, and treads in the use of medicinal leeches throughout history. British Journal of Oral Maxillofac Surgery. 2004;42: 133-137. Ref.: https://goo.gl/Wq2V38
  5. Srivastava A, Sharma R. A brief review on application of leech therapy. Arch Apll Sci Res. 2010; 2: 271-274. Ref.: https://goo.gl/YZdnmq
  6. Porshinsky B.S, Saha S, Grossman M.D, Beeryl P.R, Stawicki S.P. Clinical uses of the medicinal leech; a practical review. J Postgrad Med. 2011; 57: 65-71. Ref.: https://goo.gl/827iCk
  7. Abdualkader M, Ghawi A, Alaama M, Awang M, Merzouk A. Leech therapeutic applications. Indian J Pharm Sci. 2013; 75: 127-137. Ref.: https://goo.gl/3yWhsz
  8. National Institute of Environmental Health Sciences – NIEHS (1985). “Respect for life”.
  9. Odunlade A, Taiwo I, Arojojoye O, Lawal T. Hepatoxicity of Aqueous Leaf Extract of Bridelia ferruginea on the Liver of Albino Rats. Journal of Natural Science Research. 2015; 5: 57-64. Ref.: https://goo.gl/Z44dDK
  10. Aniagu S, Nwinyi C, Akumka D, Ajoku G, Izebe K, et al. Toxicological studies in Rats Fed Nature Cure Bitters. Afr J Biotechnol. 2005; 4: 72-78. Ref.: https://goo.gl/KDLB5m
  11. Shittu O, Lawal B, Blessing U, Haruna G, Abubakar A, et al. Alteration in Biochemical Indices Following Chronic Administration of Methanolic Extract of Nigeria Bee Propolis in Wister Rats. Asian Pacific journal of tropical disease. 2015; 5: 654-657. Ref.: https://goo.gl/RA6ptp
  12. The OECD guideline for testing of chemical. The organization of Economic co-operation development. 2001; 1-14. Ref.: https://goo.gl/y6erBL
  13. Renu S, Badri P. Screening of antibacterial activity of Hydroalcoholic extract of Cynodon dactylon. International Journal of Research in Ayurveda and Pharmacy. 2012; 3: 827-829. Ref.: https://goo.gl/rnAj9g
  14. Kabiru Y, Okogun J, Gbodi T, Makun H, Obgadoyi E. Subchronic Toxicity Studies of Methanolic Extract of Eucalyptus camaldulensis (Leaf) In Wister Rats. Iosr Journal of Pharmacy. 2013; 3: 38-45. Ref.: https://goo.gl/FH6tXp
  15. Atsafack S, Kuiate J, Mouokeu R, Koanga M, Tchinda A, et al. Toxicological studies of stem bark extract from Schefflera barteri Harms (Araliaceae). BMC Complement Alternative Medicine. 2015;7: 44. Ref.: https://goo.gl/oWsiGF
  16. Sireatawong N, Lertprasertsuke U, Scrisawat S. Acute ans sub-chronic toxicity study of the water extract from Tiliacora trianora (Colebr.) Diets in rats. Songklankarin Journal of Science and Technology. 2008; 30: 729-737. Ref.: https://goo.gl/Zj8uB2
  17. Ezeja M, Anaga A, Asuzu, I. Acute and sub-chronic toxicity profile of methanol leaf extract of Gouania longipetala in rats. Journal of Ethnopharmacology. 2014;151: 1155-1164. Ref.: https://goo.gl/ttv1Hr
  18. Sellers R, Morton D, Michael B, Roome N, Johnson J, et al. Society of Toxicologic Pathology Position Paper: Organ Weight Recommendations for Toxicologic Studies. Toxicologic Pathology. 2007; 35: 751-755. Ref.: https://goo.gl/BuqbHf
  19. Bailey S, Zidell R, Perry R. Relationships Between Organ Weight and Body/Brain Weight in the Rat: What Is The Best Analytical Endpoint? Toxicologic Pathology. 2004;32: 448-466. Ref.: https://goo.gl/CPxH1R
  20. Albert D, Telephone B, Jacques Y. Acute and subchronic oral toxicity assessment of the aqueous extract from the stem bark of Erythrina senegalensis DC (Fabaceae) in rodents. Journal of Ethnopharmacy. 2011; 134: 697-702. Ref.: https://goo.gl/PYkNpU
  21. Enechi O, Ozougwu V. Effects of ethanol extract of Mucuna pruriens leaves on the lipid profile and serum electrolytes of rats. Journal of Pharmacy and Biological Sciences. 2014;9: 18-23. Ref.: https://goo.gl/wHqjfr
  22. Olatunji I, Adebayo J, Oguntoye O, Olatunde N, Olatunji V, et al. Effects of aqueous extracts of petals of red and green Hibiscus sabdariffa on plasma lipids and haematological variables in rats. Pharmaceutical Biology. 2005; 43: 471-474. Ref.: https://goo.gl/e6BGUD
  23. EL–Demerdash F. Antioxidant effect of vitamin E and selenium on lipid peroxidation, enzyme activities and biochemical parameters in rats exposed to aluminium. Journal of Trace Elements and Medical Biology. 2004; 18: 113-122. Ref.: https://goo.gl/9Uyg6U
  24. Mbajiorgu E, Air T, Volk W, Albert M, Debusho L. Haematological Profile of Male Rats Treated with Ethanol and/or Chloroquine and fed Normal or Low Protein Diet. The Internet Journal of Hematology. 2006; 3: 1-11.
  25. Adebayo J, Adesokan A, Olatunji L, Buoro D, Soladoye A. Effect of ethanolic extract of Bougainvillea spectabilis leaves on haematological and serum lipid variables in rats. Biochemistry. 2005; 17: 45-50. Ref.: https://goo.gl/i7WZmZ
  26. Sani, D, Sanni S, Sandabe U, Ngulde S. Effect of intake of aqueous stem extract of Anisopus manni on haematilogical parameters in rats. International Journal of Applied Research in Natural products. 2009; 2: 22-28. Ref.: https://goo.gl/GQZdUR

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  • Evaluation of the effects of Leech Salivary Extract (LSE) on Haematological parameters in Rats
    Hausatu Babayi*, Ojo Olufunmilola Praise, Labake Fadipe Ajoke, Israel Kayode Olayemi, Elisha Baba and Oladosun Oluwale Peter Hausatu Babayi*,Ojo Olufunmilola Praise,Labake Fadipe Ajoke,Israel Kayode Olayemi,Elisha Baba,Oladosun Oluwale Peter. Evaluation of the effects of Leech Salivary Extract (LSE) on Haematological parameters in Rats. . 2018 doi: 10.29328/journal.jhcr.1001006; 2: 006-014

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