About University of Dschang

University of Dschang

Articles by University of Dschang

Molecular profiles of hepatotoxicity and nephrotoxicity markers in dysmenorrheic (on treatment or not) students

Published on: 18th February, 2020

OCLC Number/Unique Identifier: 8550958914

Background: Dysmenorrhea is menstrual disorder that affects about 40% - 90% of women worldwide, it is associated with oxidative stress. The current treatment of this condition is administration of non-steroidal anti-inflammatory drugs, which when frequently used, may affect organs. Objective: Assess the hepatotoxicity and nephrotoxicity side effects related to dysmenorrhea and its treatment Materials and methods: A survey (questionnaire) was designed and implemented on 689 female students of the University of Dschang. After this, and following the inclusion criteria, 191 blood samples were collected for assay of hepatotoxicity markers (transaminases, albumin), nephrotoxicity indicators (creatinine, urea, total protein) and the inflammation associated indicators. The measurements were performed on fully automated Olympus AU 400 Analyzer, using standard reagent kits. Results: Subjects with untreated dymenorrhea lasting more than five years had a significantly high level (p < 0.05) of ALT (39.47 ± 15.74 IU/L) and AST (44.37 ± 13.74 IU/L). Transaminases levels were significantly associate (p < 0.01) and positively correlate (0.251 for ALT and 0. 223 for AST) with the disease duration. Dysmenorrheic individuals on medication for more than 9 years had significantly higher ALT (25.14 ± 7.85 IU/L) and AST (35.26 ± 0.70 IU/L) levels (p < 0.05) compared to those under treatment for less than 5 years (19.37 ± 8.27 UI/L and 27.68 ± 8.56 UI/L). The use of analgesics, regardless of the duration of treatment, had normal creatinine clearance (107.44 ± 30.86 ml/min), compared to those treated with either anti-inflammatory drugs (71.56 ± 26.44 ml/min), or a combination of analgesics and anti-inflammatory drugs (81.34 ± 31.97 ml/min), which was significantly reduced (p < 0.05). Conclusion: Dysmenorrhea duration, type and duration of treatment potentially expose participants to liver and kidney disorders.
Cite this ArticleCrossMarkPublonsHarvard Library HOLLISGrowKudosResearchGateBase SearchOAI PMHAcademic MicrosoftScilitSemantic ScholarUniversite de ParisUW LibrariesSJSU King LibrarySJSU King LibraryNUS LibraryMcGillDET KGL BIBLiOTEKJCU DiscoveryUniversidad De LimaWorldCatVU on WorldCat

Stability Duration of a Normal Control Pool for Prothrombin Time and Activated Partial Thromboplastin Time Prepared in the Hematology and Blood Bank Service of the Yaoundé University Teaching Hospital, Cameroon

Published on: 10th November, 2025

Prothrombin Time (PT), also called Quick’s Time (QT), is a coagulation test expressed in seconds, measured in the presence of tissue thromboplastin and calcium ions. Similarly, Activated Partial Thromboplastin Time (APTT) is measured in the presence of phospholipids, an activator, and calcium ions. These two tests are among the most commonly used in hemostasis. Their execution absolutely requires plasma samples used as quality controls, whether commercial or prepared locally (“pooled” in the laboratory).This descriptive study aimed to determine the stability duration of an in-house control pool for PT and APTT tests, prepared in the laboratory of the Hematology and Blood Bank service of the Yaoundé University Teaching Hospital, Cameroon.Plasma was collected after centrifugation, mixed, and then frozen at a temperature of -30 °C to preserve the coagulation factors, especially the most labile ones. The plasma pool was then aliquoted, stored at -30 °C protected from light in a domestic freezer, and kept for a period of two weeks. A portion of the pool was tested within four hours of collection: 30 PT and APTT analyses were performed.The assay was performed manually using the tube tilt method. The means obtained were 12.71 seconds for PT and 30.66 seconds for APTT. The acceptability limits (mean ± 2 standard deviations), calculated from the standard deviation, were 11.38 to 14.04 seconds for PT and 27.96 to 33.37 seconds for APTT.The plasma pool was then analyzed daily in duplicate. The average of the results for each day was plotted on a Levey-Jennings chart and interpreted according to Westgard rules. The results showed that the stability duration of the normal control pool is eight (8) days for PT and seven (7) days for APTT.In a context marked by a recurrent shortage of quality control reagents, for various logistical and economic reasons, these results offer a reliable alternative to commercial control samples, which are often expensive or unavailable. The use of locally prepared control pools could thus compensate for stock-outs of normal or pathological control reagents supplied by manufacturers.
Cite this ArticleCrossMarkPublonsHarvard Library HOLLISGrowKudosResearchGateBase SearchOAI PMHAcademic MicrosoftScilitSemantic ScholarUniversite de ParisUW LibrariesSJSU King LibrarySJSU King LibraryNUS LibraryMcGillDET KGL BIBLiOTEKJCU DiscoveryUniversidad De LimaWorldCatVU on WorldCat
Help ?